---
title: "Layout Plot"
output: rmarkdown::html_vignette
vignette: >
%\VignetteIndexEntry{Layout Plot}
%\VignetteEngine{knitr::rmarkdown}
%\VignetteEncoding{UTF-8}
---
```{r, include = FALSE}
knitr::opts_chunk$set(
collapse = TRUE,
comment = "#>"
)
```
The `ggalign` package offers several `align_*` functions that allow you to
precisely control plot layout and integrate additional plots. Currently, there
are two primary `align_*` functions for adding plots:
- `align_gg()`/`ggalign()`: Create ggplot object with a customized data.
- `align_panel()`/`ggpanel()`: Create ggplot object with the layout panel
data.
```{r setup}
library(ggalign)
```
```{r setup_data}
set.seed(123)
small_mat <- matrix(rnorm(81), nrow = 9)
rownames(small_mat) <- paste0("row", seq_len(nrow(small_mat)))
colnames(small_mat) <- paste0("column", seq_len(ncol(small_mat)))
```
## `align_gg`
`align_gg()` is similar to `ggplot` in that it initializes a `ggplot` data and
`mapping`. Same with other `align_*` functions. `align_gg()` allowing you to
provide data in various formats, including matrices, data frames, or simple
vectors. This data can be also inherited from the layout.
`align_gg()` always applies a default mapping for the axis of the data index in
the layout. This mapping is `aes(y = .data$.y)` for horizontal stacking
(including left and right heatmap annotation) and `aes(x = .data$.x)` for
vertical stacking (including top and bottom heatmap annotation). For more
information, refer to the "ggplot2 Specification" section in the `align_gg()`
documentation.
You can also use the `ggalign()` function, which is an alias for `align_gg()`.
```{r}
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t") +
ggalign(data = rowSums) +
geom_point(aes(y = value))
```
### Plot data
`align_gg()`/`ggalign()` requires the specific data format for its operations.
If you need to transform or filter data for individual `geoms`, you can use the
`data` argument within each `geom`. However, if you have multiple `geoms` and
want a consistent transformation applied across all, you can utilize the plot
action argument `data` in the `align_gg()`/`ggalign()` function. This allows you
to transform the default data for all subsequent geoms.
```{r}
set.seed(1234L)
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t") +
align_kmeans(3L) +
ggalign(action = plot_action(data = function(data) subset(data, .panel == 1L))) +
geom_bar(aes(y = value, fill = .row_names), stat = "identity")
```
If the action data function has been set in both the single plot and the parent
layout, Whether the action data function is applied after the parent layout's
action data depends on the `inherit` argument in `plot_action()`. By default,
this argument is set to `FALSE`, meaning that the action data function will not
apply the transformations from the parent layout.
The following plot will result in an empty plot area since no data remains after
filtering:
```{r}
set.seed(1234L)
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t",
action = plot_action(data = function(data) subset(data, .panel == 1L))
) +
align_kmeans(3L) +
# this action data function will be applied after the layout action data
# function, so there is no panel in `2`
ggalign(action = plot_action(
data = function(data) subset(data, .panel == 2L),
inherit = TRUE
)) +
geom_bar(aes(y = value, fill = .row_names), stat = "identity")
```
To resolve this issue, you can set `inherit = FALSE`, or keep the default value:
```{r}
set.seed(1234L)
ggheatmap(small_mat) +
scale_fill_viridis_c(guide = "none") +
hmanno("t",
action = plot_action(data = function(data) subset(data, .panel == 1L))
) +
align_kmeans(3L) +
# this action data function will be applied after the layout action data
# function
ggalign(
action = plot_action(
data = function(data) subset(data, .panel == 2L),
inherit = FALSE
)
) +
geom_bar(aes(y = value, fill = .row_names), stat = "identity")
```
This functionality is particularly useful for `ggoncoplot()`, as it relies on
action data to prepare the data internally.
### Cross panel sumamry
When used in a heatmap layout, and the data is inherited from the heatmap data,
a special column `.extra_panel` will be added, which is the panel information
for column (left or right annotation) or row (top or bottom annotation). This is
useful if you want to create summary plot using another axis panel. In such
cases, it's often necessary to disable the automatic setting of limits (`limits
= FALSE` in `align_gg()`).
```{r fig.dim = c(5, 10)}
set.seed(1L)
v <- stats::rnorm(50L)
split <- sample(letters[1:2], 50L, replace = TRUE)
ggheatmap(v) +
scale_fill_viridis_c() +
theme(strip.text = element_text(), strip.background = element_rect()) +
hmanno("r") +
align_group(split) +
hmanno("t", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(.extra_panel, value, fill = .extra_panel),
# here, we use `print()` to show the underlying data
data = function(data) {
print(head(data))
data
}
) +
scale_fill_brewer(palette = "Dark2", name = "branch")
```
This approach replicates the functionality of
[ComplexHeatmap::anno_summary()](https://jokergoo.github.io/ComplexHeatmap-reference/book/heatmap-annotations.html#summary-annotation),
but is versatile enough to be used with any heatmap, not just single-column or
single-row heatmaps.
```{r}
ggheatmap(small_mat) +
theme(axis.text.x = element_text(angle = -60, hjust = 0)) +
hmanno("t") +
align_dendro(aes(color = branch), k = 3L) +
scale_color_brewer(palette = "Dark2") +
hmanno("r", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(y = .extra_panel, x = value, fill = factor(.extra_panel))) +
scale_fill_brewer(palette = "Dark2", name = "branch")
```
### Plot titles
`ggplot2` only allow add titles in the top or add caption in the bottom.
The ggalign package extends this capability, allowing you to place titles around
any border of the plot using the `patch_titles()` function.
```{r}
ggheatmap(small_mat) +
patch_titles(left = "left patch title", bottom = "bottom patch title") +
theme(axis.text.x = element_text(angle = -60, hjust = 0)) +
hmanno("t") +
align_dendro(aes(color = branch), k = 3L) +
scale_color_brewer(palette = "Dark2") +
patch_titles(top = "top patch title") +
hmanno("r", size = 0.5) +
ggalign(limits = FALSE) +
geom_boxplot(aes(y = .extra_panel, x = value, fill = factor(.extra_panel))) +
scale_fill_brewer(palette = "Dark2", name = "branch") +
patch_titles(right = "right patch title")
```
## `align_panel`
The `align_panel()` function is similar to align_gg(), but it operates
specifically with layout panel data, removing all axis labels and ticks. The
`ggpanel()` function is an alias for `align_panel()`.
The data in the underlying `ggplot` object contains following columns:
- `.panel`: the panel for current layout axis.
- `.index`: the index of the original layout data.
- `.x` or `.y`: the `x` or `y` coordinates
You can use `align_panel()` to integrate additional elements, such as block
annotation or customized panel title, into your layout.
```{r}
ggheatmap(small_mat) +
hmanno("t", size = unit(1, "cm")) +
align_kmeans(centers = 3L) +
ggpanel() +
geom_tile(aes(y = 1L, fill = .panel, color = .panel),
width = 1L, height = 1L
) +
geom_text(aes(y = 1L, label = .panel),
data = function(data) {
aggregate(.x ~ .panel, data, FUN = median)
}
)
```
## Session information
```{r}
sessionInfo()
```